Journal: International Journal of Molecular Medicine

Article Title: BMS-345541 inhibits airway inflammation and epithelial-mesenchymal transition in airway remodeling of asthmatic mice

doi: 10.3892/ijmm.2018.3762

Figure Lengend Snippet: BMS-345541 inhibits epithelial-mesenchymal transition in asthma. mRNA levels of E-cadherin and vimentin in mouse lung tissues were measured by (A) reverse transcription-quantitative polymerase chain reaction analysis. (B) Protein levels were measured by western blot analysis for (C) E-cadherin and (D) vimentin. Data are presented as the mean ± standard deviation (n=8 per group). # P<0.01 and ## P<0.001, OVA group compared with the control group; * P<0.05 and ** P<0.01, OVA + BMS-345541 group compared with the OVA group. OVA, ovalbumin; DMSO, dimethyl sulfoxide.

Article Snippet: The reagents used were as follows: OVA (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany), acetylcholine chloride (Ach; Sigma-Aldrich; Merck KGaA), phosphate-buffered saline (PBS; Sigma-Aldrich; Merck KGaA), sodium pentobarbital (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China), BMS-345541 (Abcam, Cambridge, MA, USA), E-cadherin antibody (cat. no. ab76055; Abcam), vimentin antibody (cat. no. ab92547, Abcam), β-actin antibody (cat. no. ab179467; Abcam), glyceraldehyde 3-phosphate dehydrogenase (GADPH; Sigma-Aldrich; Merck KGaA), dimethyl sulfoxide (DMSO; Sigma-Aldrich; Merck KGaA), diaminobenzidine (DAB; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), an enzyme-linked immunosorbent assay (ELISA) kit (Bio-Rad Laboratories, Inc., Hercules, CA, USA), Pierce Enhanced Chemiluminescence (ECL) western blot substrate (Thermo Fisher Scientific, Inc., Waltham, MA, USA), a reverse transcription kit (Promega Corporation, Madison, WI, USA), a quantitative polymerase chain reaction (qPCR) kit (TransStart Green; Beijing Transgen Biotech Co., Ltd., Beijing, China), a bicinchoninic acid (BCA) protein assay kit and radioimmunoprecipitation assay (RIPA) lysis buffer (Beijing ComWin Biotech Co., Ltd., Beijing, China).

Techniques: Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot, Standard Deviation, Control

Journal: FEBS Open Bio

Article Title: Generation of universal natural killer cells from a cryopreserved cord blood mononuclear cell‐derived induced pluripotent stem cell library

doi: 10.1002/2211-5463.13460

Figure Lengend Snippet: Differentiation capability of cryoCB‐iPSCs. (A) Trilineage differentiation quantitative PCR array of cryoCB‐iPSCs. (B) Representative images of immunofluorescence staining of markers of different lineage cells: AFP3 for mesoderm, 1A4 for endoderm, and 10C2 for ectoderm. Scale bar, 50 μm. (C) Representative images of H&E staining of teratomas containing endoderm, mesoderm, and ectoderm. Scale bar, 100 mm.

Article Snippet: The Human Pluripotent Stem Cell Trilineage Differentiation quantitative polymerase chain reaction (qPCR) Array (Stemcell; 7515) was performed to characterize hPSCs and their trilineage differentiation capacity.

Techniques: Real-time Polymerase Chain Reaction, Immunofluorescence, Staining